Hu Recom NGF-beta, 20 µg

STEMCELL Technologies supplies a human recombinant NGF-beta (nerve growth factor beta) preparation intended for use as a laboratory reagent. The supplier identified on the safety documentation is STEMCELL Technologies Canada Inc., based in Vancouver, British Columbia, Canada.

According to the manufacturer's product information, NGF-beta has a role in the survival and growth of neural cells, regulating cell growth, promoting differentiation into neurons, and neuron migration. The biological activity of the recombinant protein was tested by its ability to promote the proliferation of TF-1 cells grown in GM-CSF free medium; cell proliferation was measured after 67 hours of culture using a fluorometric assay method, with an example EC50 of 0.54 ng/mL.

When 1 μg of the protein was resolved by SDS-PAGE under reducing and non-reducing conditions and visualised by Coomassie Blue staining, it appeared as a homodimer of 13.6 kDa subunits with a predicted total molecular mass of 27.3 kDa. The material is supplied as a solid and is not classified as a hazardous substance or mixture according to the Globally Harmonized System (GHS) or the 2012 OSHA Hazard Communication Standard.