Hu Recom IL-9, 500 µg

Recombinant human Interleukin-9 (IL-9) is a pleiotropic cytokine used in immunology and cell biology research, including studies of T helper 9 (Th9) cell differentiation, mast cell activation, allergic inflammation, B cell class switching, and parasitic infection models. Manufactured by STEMCELL Technologies as a 500 µg lyophilized powder produced in E. coli, this research-grade reagent is bioactivity-tested and supports reproducible workflows in serum-free or defined media cultures, with endotoxin levels controlled to minimize background activation in sensitive assays.

The vial is intended for repeated reconstitution, allowing flexible dosing across multiple experimental setups ranging from dose-response titrations to long-term differentiation protocols. Researchers working on asthma, atopic dermatitis, or hematopoietic regulation use this cytokine to supplement culture systems and to probe IL-9R signaling pathways through flow cytometry, western blot detection of STAT1, STAT3, and STAT5 phosphorylation, and quantification of downstream gene expression.

Each lot is verified for purity by SDS-PAGE and HPLC analysis, with bioactivity confirmed in a cell-based proliferation assay using factor-dependent hematopoietic cell lines. Reconstitute in sterile water or appropriate buffer, store reconstituted aliquots frozen at -20 °C or below, and avoid repeated freeze-thaw cycles to preserve full activity over multi-week experiments.