Hu Recom IL-7, ACF, 1000 µg

STEMCELL Technologies supplies Human Recombinant IL-7 in an animal component-free (ACF) format, designated for use as a laboratory reagent. Interleukin 7 (IL-7) is described as a member of the type I cytokine family that is critical for T and B cell development and survival. It is produced by non-hematopoietic cells in the thymus, lymphoid organs, and by bone marrow stromal cells. IL-7 binds to a receptor (IL-7R) composed of the common gamma chain and IL-7Rα (CD127) and signals through the JAK/STAT and PI3K pathways. It regulates the survival of naïve and memory CD4+ and CD8+ T cells, γδ T cells, NK T cells, innate lymphoid cells, and regulatory T cells. Although a deficiency in IL-7R still permits the generation of normal numbers of peripheral B cells in humans, stimulation of human B cell precursors with IL-7 can promote STAT5-dependent proliferation and survival in vitro. Alternative names listed include Interleukin-7, LP-1, Lymphopoietin 1, and Pre-B cell factor, with UniProt accession P13232.

The product is expressed in E. coli and is supplied as a lyophilized solid from a sterile-filtered solution containing acetic acid. Reported specifications include a purity of ≥ 95%, a predicted molecular mass of 17.5 kDa, and an endotoxin level of ≤ 1 EU/μg protein as measured by kinetic Limulus amebocyte lysate (LAL) analysis. Specific activity is given as ≥ 1.0 x 10^6 units/mg with an EC50 ≤ 1 ng/mL, determined by a cell proliferation assay using 2E8 cells. Cross-reactivity is reported to be species-specific.

For preparation, the vial should be centrifuged before opening and reconstituted in sterile water to at least 0.1 mg/mL by pipetting the solution down the sides of the vial without vortexing. Storage is recommended at -20°C to -80°C, with the product stable as supplied for 12 months from the date of receipt. The accompanying safety data sheet classifies the material as not a hazardous substance or mixture according to the Globally Harmonized System (GHS), and it is listed as not regulated for transport by DOT, TDG, MEX, ICAO, IATA, IMDG, RID, ADR, and ADN. STEMCELL Technologies' quality management system is certified to ISO 13485, and the product is for research use only, not intended for human or animal diagnostic or therapeutic uses unless otherwise stated.