Hu Recom IL-7, 500 µg

A lyophilized preparation of human recombinant Interleukin-7 (IL-7) is supplied by STEMCELL Technologies. IL-7 is a member of the type I cytokine family that is critical for T and B cell development and survival. It is produced by non-hematopoietic cells in the thymus and lymphoid organs, and by bone marrow stromal cells. The cytokine binds to a receptor (IL-7R) composed of the common gamma chain and IL-7Rα (CD127) and signals through the JAK/STAT and PI3K pathways. It regulates the survival of naïve and memory CD4+ and CD8+ T cells, γ/δ T cells, NKT cells, innate lymphoid cells, and regulatory T cells.

Species: Human; produced in E. coli. The product is lyophilized from a sterile-filtered aqueous solution containing sodium phosphate at pH 7.5. Alternative names include Interleukin-7, LP-1, Lymphopoietin 1, and Pre-B cell factor (UniProt accession P13232). Purity is ≥ 97%. Specific activity is ≥ 2 x 10^6 units/mg, corresponding to an EC50 ≤ 0.5 ng/mL, as determined by a cell proliferation assay using phytohemagglutinin (PHA)-stimulated human peripheral blood mononuclear cells (PBMCs). Endotoxin level, measured by kinetic Limulus amebocyte lysate (LAL) analysis, is ≤ 1 EU/μg protein.

Store at -20 to -80°C; stable as supplied for 12 months from date of receipt. Centrifuge the vial before opening and reconstitute in sterile water to at least 0.1 mg/mL by pipetting the solution down the sides of the vial; do not vortex. For research use only.