Hu Recom IL-7, 1000 µg

STEMCELL Technologies offers a recombinant human Interleukin-7 (IL-7) protein expressed in E. coli and supplied as a lyophilized preparation from a sterile-filtered aqueous solution containing sodium phosphate, pH 7.5. IL-7 is a member of the type I cytokine family that is critical for T and B cell development and survival, and is produced by non-hematopoietic cells in the thymus and lymphoid organs and by bone marrow stromal cells. It binds to a receptor (IL-7R) composed of common gamma chain and IL-7Rα (CD127) and signals through the JAK/STAT and PI3K pathways. The cytokine regulates the survival of naïve and memory CD4+ and CD8+ T cells, γ/δ T cells, NKT cells, innate lymphoid cells, and regulatory T cells. Stimulation of human B cell precursors with IL-7 can promote STAT5-dependent proliferation and survival in vitro.

The protein has a predicted molecular mass of 17.5 kDa and a purity of ≥ 97%. Specific activity is ≥ 2 x 10^6 units/mg, with an EC50 of ≤ 0.5 ng/mL as determined by a cell proliferation assay using phytohemagglutinin (PHA)-stimulated human peripheral blood mononuclear cells (PBMCs). Endotoxin level is ≤ 1 EU/μg protein, as measured by kinetic Limulus amebocyte lysate (LAL) analysis. Alternative names for this protein include Interleukin-7, LP-1, Lymphopoietin 1, and Pre-B cell factor, with UniProt accession number P13232. The product should be stored at -20 to -80°C and is stable as supplied for 12 months from the date of receipt. For research use only.