Hu Recom IL-4, 100 µg

Supplied by STEMCELL Technologies, this recombinant preparation of human interleukin-4 (IL-4) is intended for use as a laboratory reagent. The manufacturer describes IL-4 as a cytokine that drives immunoglobulin class switching in B cells, mast cell hyperplasia, mucus production, and differentiation of naïve T cells into Th2 cells.

Biological activity of the preparation is tested by its ability to promote the proliferation of TF-1 cells, with cell proliferation measured after 72 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum, and the EC50 in the assay example is 0.16 - 0.24 ng/mL. The product has a predicted molecular mass of 15.1 kDa, and 1 μg of Human Recombinant IL-4 was resolved with SDS-PAGE under reducing and non-reducing conditions and visualized by Coomassie Blue staining.

According to the supplier's safety data sheet, the material is a solid and is not classified as hazardous by the 2012 OSHA Hazard Communication Standard (29 CFR 1910.1200), nor is it a hazardous substance or mixture according to the Globally Harmonized System (GHS). The supplier, STEMCELL Technologies Canada Inc., is based in Vancouver, British Columbia, Canada, and storage should follow the conditions listed on the Product Information Sheet.