Hu Recom IL-17A, 100 µg

From STEMCELL Technologies, Human Recombinant IL-17A is the founding member of the IL-17 cytokine family, which includes IL-17B through IL-17F. It acts as a potent pro-inflammatory cytokine that plays a key role in defense against pathogens.

IL-17A mediates protection against extracellular pathogens and induces granulopoiesis factors and neutrophil-specific chemokines. It is produced by Th17 cells, CD8+ T cells, γ/δ T cells, natural killer (NK) T cells, B cells, innate lymphoid cells, and mesenchymal stromal cells (MSCs). IL-17A and IL-17F signal as homodimers or heterodimers through the same receptor and activate NF-κB, MAPK, and C/EBP pathways. Together with IL-22, IL-17A stimulates production of antimicrobial peptides, and together with tumor necrosis factor alpha (TNF-α), it induces a sustained neutrophil recruitment during inflammation. The IL-17A receptor is expressed at particularly high levels on stromal cells, including MSCs. IL-17A increases the frequency and average size of fibroblast colony-forming units (CFU-F), as well as the proliferation of marrow-derived MSCs. It enhances osteogenic differentiation, and inhibits adipocyte differentiation and chondrogenesis.

Biological activity of Human Recombinant IL-17A was tested by its ability to produce IL-6 in NIH 3T3 cells. Production of mouse IL-6 was measured after 48 hours of culture, with an EC50 of 2.7 ng/mL. The protein is a homodimer of 15.7 kDa subunits with a predicted total molecular mass of 31.3 kDa. 1 µg of Human Recombinant IL-17A resolved with SDS-PAGE under reducing and non-reducing conditions was visualized by Coomassie Blue staining.