Hu Recom IGF-I, ACF, 1000 µg

A recombinant polypeptide supplied by STEMCELL Technologies in an animal component-free formulation, Insulin-like Growth Factor 1 (IGF-I) binds to the IGF-I receptor and is a potent activator of the PI3K/AKT pathway, also activating ERK1/2 signaling. IGF-I is required for embryonic development and is produced mainly in the liver in response to a hepatocyte growth hormone. In the absence of insulin, IGF-I is necessary for the maintenance of human pluripotent stem cells. Together with IL-3, IGF-I stimulates differentiation and proliferation of myeloid cells and regulates lymphopoiesis by stimulating proliferation and differentiation of T and B cells in lymphoid organs.

Also known by alternative names including IBP1, IGF-IA, IGF-IB, IGF1A, Mechano growth factor (MGF), and Somatomedin C (UniProt Accession P05019), the recombinant human protein is expressed in E. coli and lyophilized from a sterile-filtered aqueous solution containing 0.1% trifluoroacetic acid. Purity is ≥ 95% as determined by SDS-PAGE. Specific activity is ≥ 6.7 x 10^5 units/mg with an EC50 ≤ 1.5 ng/mL, as determined by a cell proliferation assay using MCF-7 cells. Endotoxin level is ≤ 0.1 EU/μg protein as measured by kinetic Limulus amebocyte lysate (LAL) analysis. Store at -20 to -80 °C; the product is stable as supplied for 12 months from date of receipt. Reconstitute in sterile water to at least 0.1 mg/mL by pipetting the solution down the sides of the vial; do not vortex.

According to the manufacturer's safety data sheet, this product is not classified as a hazardous substance or mixture according to the Globally Harmonized System (GHS) or the 2012 OSHA Hazard Communication Standard, and is supplied as a solid. For research use only.