Hu Recom IFN-gamma, ACF, 1000 µg

An animal component-free preparation of type II interferon from STEMCELL Technologies, this recombinant human cytokine is produced by T and NK cells—and in smaller amounts by dendritic cells and macrophages—with its expression controlled by cytokines such as IL-12 and IL-18 secreted in response to infection. IFN-γ stimulates the antimicrobial and anti-tumor activity of macrophages, NK cells, and neutrophils by promoting microbial effector functions such as the production of reactive oxygen species, nitric oxide intermediates, and complement, and enhances major histocompatibility complex class I and II expression on dendritic cells and mononuclear phagocytes.

Expressed in E. coli, the protein has a predicted molecular mass of 16.9 kDa and a purity of ≥ 95%. Specific activity is ≥ 3.3 x 10^6 units/mg (≤ 0.3 ng/mL), as determined by the ability to induce cytotoxicity of HT-29 cells, and endotoxin level is ≤ 0.1 EU/μg protein. The product is supplied as a lyophilized preparation from a sterile-filtered aqueous solution containing sodium phosphate, trehalose, mannitol, and TWEEN 80 at pH 6.5, and should be stored at -20 to -80°C.

This reagent is intended for research use only and not for human or animal diagnostic or therapeutic uses unless otherwise stated.