Hu Recom Heregulin-beta 1, 1000 µg

STEMCELL Technologies offers this laboratory reagent as a research-grade solid. Human Recombinant Heregulin-beta 1 is described by the manufacturer as modulating cell growth and differentiation during development and wound healing, and promoting metastasis of breast tumor cells.

Biological activity is demonstrated by its ability to promote the proliferation of MCF-7 cells, measured using a fluorometric assay. The EC50, defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum, is less than 0.1 ng/mL. The protein has a predicted molecular mass of 7.5 kDa and has been resolved with SDS-PAGE under reducing (+) and non-reducing (−) conditions and visualized by Coomassie Blue staining. It is featured within the PI3K/AKT pathway research area.

The manufacturer's safety data sheet classifies this material as not hazardous according to the Globally Harmonized System (GHS) and the 2012 OSHA Hazard Communication Standard (29 CFR 1910.1200). Recommended storage is frozen at −20 °C, with aliquoting after reconstitution to prevent freeze–thaw damage. Third-party guidance specifies reconstitution with sterile water or PBS containing 0.1% BSA. Reported applications include large-scale cell culture, receptor activation assays, and cardiac and neuronal differentiation studies. For research use only.