Hu Recom Fractalkine (CX3CL1), 5 µg

From STEMCELL Technologies, this recombinant chemokine supports research in immunology and stem cell biology. Fractalkine (CX3CL1) functions as a potent chemoattractant of target cells and has been implicated in the pathology of inflammatory diseases.

Recombinant cytokines from STEMCELL Technologies are produced under stringent criteria, with a guaranteed purity of ≥ 95% and endotoxin levels of ≤ 0.2 EU/µg protein to support consistency and reproducibility across multiple applications. The reagent is featured in research workflows that include the differentiation of human monocytes into dendritic cells and the immunomagnetic negative isolation of untouched human T cells.

Biological activity was assessed by the ability of the protein to mobilize Ca2+ in CHO-K1/Gα15/hCX3CR1 cells, measured using a fluorometric assay. The EC50, defined as the effective concentration at which Ca2+ mobilization is at 50% of maximum, is less than 1.5 µg/mL. On SDS-PAGE under reducing (+) and non-reducing (-) conditions with Coomassie Blue staining, the protein exhibits a predicted molecular mass of 50 - 75 kDa.