Hu Recom CNTF, ACF, 20 µg

Manufactured by STEMCELL Technologies, this Human Recombinant CNTF, ACF is an animal component-free cytokine intended for stem cell research workflows. Ciliary neurotrophic factor (CNTF) supports neurogenesis and regulates the survival and differentiation of neural stem cells. The product is positioned for use in research areas involving the neural and pancreatic differentiation of mouse and human ES and iPS cells, where it is used alongside related animal component-free supplements such as brain-derived neurotrophic factor, basic fibroblast growth factor, and epidermal growth factor.

Biological activity is determined by the protein's ability to promote proliferation of TF-1 cells, with cell proliferation measured using a fluorometric assay method. The EC50 is defined as the effective concentration at which proliferation reaches 50% of maximum, and in the manufacturer's example assay this value is 96.7 ng/mL. The protein has a predicted molecular mass of 22.9 kDa, as visualised by SDS-PAGE under reducing and non-reducing conditions followed by Coomassie Blue staining.

The product falls within the cytokines category, specifically classified as a human cytokine.