Hu Recom CNTF, ACF, 100 µg

STEMCELL Technologies offers this animal component-free preparation of human ciliary neurotrophic factor (CNTF), a neurotrophic factor belonging to the four-helix bundle cytokine family. CNTF is structurally related to interleukin 6 (IL-6), interleukin 11 (IL-11), leukemia inhibitory factor (LIF), and oncostatin M (OSM). The factor binds to its receptor CNFTRα and induces formation of a heterodimer of the signal-transducing IL-6 receptor gp130 and LIF receptor (LIFR)-β, which triggers JAK/STAT, ERK, and PI3K signaling cascades.

CNTF plays an important role in neurogenesis and the differentiation of neural stem cells and has been suggested to possess a therapeutic role in treating neurological disorders. It has also been shown to protect rod photoreceptors from light-induced damage and to have therapeutic effects on retinal degenerative diseases caused by genetic defects or damage induced by toxins, autoantibodies, or strong light. Another therapeutic role of CNTF has been reported in protecting oligodendrocytes from apoptosis-induced death. Additionally, CNTF is commonly used to differentiate human pluripotent stem cell (hPSC)-derived neural progenitor cells into astrocytes. The product is reported to be species-specific.

The protein is produced in E. coli and has a predicted molecular mass of 22.9 kDa, with a purity of ≥ 95% as determined by SDS-PAGE. Endotoxin levels are ≤ 1 EU/μg protein as measured by kinetic Limulus amebocyte lysate (LAL) analysis. Specific activity is ≥ 3.1 x 10^3 units/mg (EC50 ≤ 325 ng/mL) as determined by a cell proliferation assay using TF-1 cells. The UniProt accession number is P26441. The lyophilized product is prepared from a sterile-filtered solution containing sodium phosphate, pH 7.5, and should be reconstituted in sterile water to at least 0.1 mg/mL. Store at -20°C to -80°C; stable as supplied for 12 months from date of receipt. This product is for research use only and is not intended for human or animal diagnostic or therapeutic uses. STEMCELL Technologies' quality management system is certified to ISO 13485.