Hu Recom BMP-4, 100 µg

Bone morphogenetic protein 4 (BMP-4) is a member of the highly conserved transforming growth factor β (TGF-β) superfamily of signaling molecules. Mature BMP-4 is a disulfide-linked homodimeric protein consisting of two 116-amino-acid residue subunits, generated by proteolytic removal of the signal peptide and propeptides. The protein binds to type I and type II receptors on cells, triggering phosphorylation of receptor 1 and downstream Smad proteins that then act as transcription factors.

BMPs are key regulators of embryogenesis and play a role in the growth and differentiation of various cell types, including embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), mesenchymal cells, epithelial cells, hematopoietic cells, and neuronal cells. Alternative names include BMP-2B, BMP2B1, MCOPS6, OFC11, and ZYME, with UniProt Accession Number P12644.

Manufactured by STEMCELL Technologies, this human recombinant protein is expressed in CHO cells and supplied as a lyophilized powder from a sterile-filtered solution containing 0.1% trifluoroacetic acid. The specific activity is ≥ 6.7 x 10^4 units/mg (EC50 ≤ 15 ng/mL), determined by alkaline phosphatase activity induced in ATDC-5 cells; a representative assay yielded an EC50 of 3.99 ng/mL. Purity is ≥ 95% and endotoxin level is ≤ 1 EU/μg protein as measured by kinetic Limulus amebocyte lysate (LAL) analysis. The predicted molecular mass is 13.1 kDa (monomer) or 26.2 kDa (dimer); on SDS-PAGE, the protein migrates to an apparent molecular mass of 20 kDa or 34 kDa due to variable glycosylation. The product should be stored at -20 to -80°C and reconstituted in 10 mM sterile hydrochloric acid to at least 0.1 mg/mL.