Hu Recom BDNF, 10 µg

A recombinant human brain-derived neurotrophic factor (BDNF) from STEMCELL Technologies supports the survival and differentiation of specific neuronal subpopulations in the central and the peripheral nervous systems. Biological activity was tested by its ability to promote the proliferation of a neuroblastoma cell line stably expressing TrkB (BR6), with cell proliferation measured using a colorimetric assay method. The EC50 in this assay is 5 ng/mL. The protein is a homodimer of 13.6 kDa subunits with a predicted molecular mass of 27.3 kDa, demonstrated by resolving 1 μg with SDS-PAGE under reducing and non-reducing conditions and visualizing by Coomassie Blue staining.