Hu IL-1RA, ACF, 1000 _g

Recombinant Human IL-1 Receptor Antagonist (IL-1RA) expressed and manufactured under animal component-free conditions, supplied by STEMCELL Technologies as a 1000 µg vial intended for in vitro research use. This naturally secreted cytokine antagonist binds competitively to the IL-1 receptor, blocking downstream signaling from both IL-1α and IL-1β without triggering pro-inflammatory cascades. In hematopoietic and pluripotent stem cell workflows, IL-1RA is supplemented into culture media to suppress unintended myeloid bias and to support controlled lineage commitment during embryoid body formation or directed differentiation protocols.

The animal component-free formulation reduces lot-to-lot variability introduced by serum- or bovine-derived contaminants, supporting reproducible expansion of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). Researchers reconstitute the 1000 µg vial in sterile PBS or serum-free culture medium and titrate into established protocols; preparing single-use aliquots minimizes repeated freeze-thaw of the stock solution. Working concentrations generally span the low ng/mL range depending on cell type and application. The vial is shipped on dry ice and stored at -20°C or colder for long-term stability, with thawed portions held at refrigerated temperatures for short-term use only.