Hu IL-1RA, ACF, 100 _g

STEMCELL Technologies offers Human Recombinant IL-1RA in an Animal Component Free (ACF) formulation. This recombinant human interleukin-1 receptor antagonist functions as a natural regulator of IL-1 biological activity, binding to IL-1 receptors with similar affinity as IL-1 itself and thereby inhibiting the proinflammatory activities of both IL-1α and IL-1β. The protein has a predicted molecular mass of 17.3 kDa, as resolved by SDS-PAGE under reducing and non-reducing conditions and visualized by Coomassie Blue staining.

The product is supplied lyophilized and should be stored at -20°C, with freeze-thaw cycles minimized. Reconstitution can be performed in sterile water or buffer, with the addition of 0.1% BSA potentially improving solubility and stability. In a cell-based proliferation assay, biological activity was assessed by the ability to inhibit human IL-1 alpha-induced proliferation of D10.G4.1 cells, yielding an EC50 of 18.7 ng/mL.

Typical applications include cytokine and chemokine pathway studies, cell culture blockade experiments, ELISA, and functional assays. The reagent is intended for research use only and is not for clinical use.