HiSpeed Plasmid Mega EF Kit (5)

QIAGEN's HiSpeed Plasmid Mega EF Kit provides vacuum-driven, large-scale, anion-exchange-based preparation of transfection-grade endotoxin-free plasmid or cosmid DNA. The kit delivers up to 2.5 mg of high-copy plasmid DNA from 500 ml of bacterial culture, with each run processing 6 samples in approximately 50 minutes through combined vacuum and centrifugation steps.

A QIAvac HiSpeed LS vacuum manifold is required for the protocol. Included with the kit are QIAfilter Cartridges, HiSpeed Tips, and QIAconcentrator modules. The vacuum-driven QIAfilter module replaces the centrifugation step after alkaline lysis, clearing bacterial lysates rapidly by removing SDS precipitates. Prepacked HiSpeed Tips operate by gravity flow and never run dry, while the QIAconcentrator captures isopropanol-precipitated DNA for elution directly into a collection tube with TE buffer or water.

Purified DNA is endotoxin-free at less than 0.04 EU/µg DNA, with purity equivalent or superior to that obtained by two successive rounds of CsCl gradient centrifugation. The kit is intended for molecular biology applications including transfection, cloning, sequencing, and plasmid-mediated gene silencing, and the resulting DNA is also suitable for gene therapy research and other sensitive applications. Kits and components should be stored dry at 15–25°C.