GSA 10, 10 mg

This Hedgehog pathway activator from STEMCELL Technologies is a small-molecule agonist of Smoothened (SMO), a cell surface receptor and mediator of the Hedgehog signaling pathway. As a quinolinecarboxamide derivative, it binds in a distinct binding pocket from cyclopamine. The compound is active at an EC₅₀ of 1.2 µM in an alkaline phosphatase-based mouse mesenchymal cell line (C3H10T1/2) differentiation assay (Gorojankina et al.). Published applications include promoting differentiation of multipotent mesenchymal progenitor cells into osteoblasts (Gorojankina et al.).

The chemical name is 4-[[(1-hexyl-1,2-dihydro-4-hydroxy-2-oxo-3-quinolinyl)carbonyl]amino]-benzoic acid, propyl ester, with CAS Number 300833-95-8, molecular formula C₂₆H₃₀N₂O₅, and molecular weight 450.5 g/mol. Purity is ≥ 95%. The compound is supplied as a crystalline solid and is soluble in DMF (≤ 2 mM), with low solubility in aqueous media. For use as a cell culture supplement, the stock solution should be diluted into culture medium immediately before use, with the final DMF concentration kept at or below 0.1% to avoid potential cell toxicity.

Product is stable at -20°C as supplied when protected from prolonged exposure to light, and is stable as supplied for 12 months from the date of receipt. The product is hazardous; please refer to the Safety Data Sheet. For research use only and not intended for human or animal diagnostic or therapeutic uses unless otherwise stated.