Gentra Puregene Buccal Cell Kit (100)

Using an improved salting-out precipitation method, the Gentra Puregene Buccal Cell Kit from QIAGEN purifies DNA without the need to mix or dilute solutions, keeping hands-on time to a minimum. Cells are lysed with an anionic detergent in the presence of a DNA stabilizer that limits the activity of intracellular and environmental DNases. RNA is removed by treatment with an RNA digestion enzyme, while other contaminants such as proteins are eliminated by salt precipitation. Genomic DNA is recovered by alcohol precipitation and dissolved in a solution of 1 mM EDTA and 10 mM Tris·Cl at pH 7.5. Purified DNA typically displays an A260/A280 ratio between 1.7 and 1.9 and can reach a size of up to 200 kb. The DNA can be safely stored at 2–8°C, –20°C, or –80°C.