EpiTect Fast 96 DNA Bisulfite Kit

From QIAGEN, this kit provides a fast and streamlined procedure for efficient bisulfite conversion and cleanup of DNA prepared from FFPE, blood, cultured cells, or tissue samples, optimized for methylation analysis in a 96-well format. During bisulfite treatment, unmethylated cytosine residues are converted to uracil while methylated cytosines remain unchanged, allowing methylation status to be analyzed by PCR, real-time PCR, methylation-specific PCR, bisulfite sequencing, COBRA, and Pyrosequencing. The kit contains a highly concentrated Bisulfite Solution that reduces the conversion time from 5 hours to as little as 30 minutes, along with preparation buffers that remove the need to isolate DNA prior to bisulfite treatment. DNA fragmentation during conversion is prevented by a unique DNA Protect Buffer, which contains a pH-indicator dye that confirms the correct pH for cytosine conversion. The bisulfite thermal cycling program delivers optimized incubation steps for thermal DNA denaturation, sulfonation, and cytosine deamination, enabling cytosine conversion rates of over 99%.

The protocol accepts high-concentration samples of 1 ng – 2 µg or low-concentration samples of 1–500 ng of DNA. The kit is shipped at room temperature (15–25°C); the DNA Protect Buffer and Buffer BD should be stored at 2–8°C upon arrival, while all other buffers, including the Bisulfite Solution, are stored at room temperature and remain stable for at least 6 months. The product is intended for molecular biology applications and is not intended for the diagnosis, prevention, or treatment of a disease.