DNeasy Plant Pro Kit (250)

QIAGEN's DNeasy Plant Pro Kit enables fast and easy purification of total cellular DNA from plant cells, tissues, and seeds, while removing polyphenolics and polysaccharides. The kit uses bead-beating technology in place of traditional CTAB, phenol, or chloroform extraction, making it suitable for challenging sample types including strawberry leaf, grapevine leaf, pine needles, and various seed types. It can also be used to purify bacterial, viral, and fungal DNA from plant and root samples. Estimated yields from 50 mg of sample include 15–23 µg for grass leaf, 7–15 µg for pine needle, and 6–14 µg for sunflower seeds.

QIAGEN's second-generation patented Inhibitor Removal Technology (IRT) removes PCR inhibitors such as polysaccharides, polyphenolics, and other secondary metabolites, delivering inhibitor-free DNA ready for immediate use in downstream applications including PCR, qPCR, RAPD analysis, RFLP analysis, Southern Blotting, and next-generation sequencing. The kit is designed to isolate high-quality DNA from 5–100 mg of even the most difficult plant samples. Cell lysis and DNA release occur by mechanical and chemical methods; released genomic DNA is captured on a silica membrane in a spin-column format, then washed and eluted.

The workflow is compatible with the TissueLyser II and the PowerLyzer 24 Homogenizer for high-velocity bead-beating, with a Vortex Adapter available for easier-to-lyse samples. Purification can be fully automated on QIAcube instruments using the same lyse, bind, wash, and elute steps as the manual procedure (the lyse step must be performed manually). Kit components include Tissue Disruption Tubes, MB Spin Columns, Buffers, and Collection Tubes (1.5 ml and 2 ml). The kit is intended for molecular biology applications and is not intended for the diagnosis, prevention, or treatment of disease; manufacturing follows QIAGEN's ISO-certified Quality Management System.