Custom qPCR LNA Assay (Probe)

Sequence-specific quantitative PCR detection relies on locked nucleic acid–modified probes to deliver tight binding affinity and reliable discrimination across closely related targets. Each order is custom-built against a user-submitted sequence, generating a tailored primer and probe set that supports applications including gene expression profiling, microRNA quantification, viral pathogen detection, and SNP genotyping. The dual-labeled hydrolysis probe is supplied in a single tube format with a 5' fluorescent reporter and 3' non-fluorescent quencher, ready for direct use in standard real-time PCR protocols. Increased thermal stability from LNA substitution permits shorter probe lengths while maintaining a high melting temperature, giving researchers confidence to distinguish single-nucleotide variants and splice isoforms. Storage at -20 °C and protection from light preserve fluorophore integrity across multiple freeze-thaw cycles when aliquoted. Compatible with research use only workflows, the reagents perform across most real-time thermal cyclers and integrate with established analysis pipelines. The custom design service from QIAGEN combines bioinformatic evaluation, sequence optimization, and quality control verification to ensure each assay meets the performance requirements of downstream experiments.