Custom miRCURY LNA Inhibitor (5)

Locked nucleic acid (LNA) antisense oligonucleotides from QIAGEN deliver potent, sequence-specific silencing of endogenous microRNAs in functional genomics research. Each custom inhibitor is built to target a user-selected miRNA sequence, supporting loss-of-function studies that dissect miRNA-mediated regulation of gene expression, validate predicted targets, and characterize pathway-level effects in both cultured cells and in vivo model systems.

The reagent is supplied as a 5 nmol, lyophilized single-use vial that the researcher reconstitutes in nuclease-free water to a working stock suitable for lipid-mediated transfection. Incorporating LNA chemistry raises target affinity and improves nuclease resistance relative to standard DNA-based antisense oligos, enabling efficient knockdown at low nanomolar working concentrations. Recommended storage is at -20 °C, protected from light, to preserve LNA integrity across multiple freeze-thaw cycles of the working stock.

Typical workflows pair the inhibitor transfection with downstream assessment of miRNA activity through reverse transcription quantitative PCR (RT-qPCR) or small RNA sequencing. The 5 nmol pack size accommodates dose-response studies, time-course experiments, and replicate treatments across multiple biological samples in cell culture and RNA interference investigations.