Custom LNA Oligo manual design (1) - 3' TAMRA-Sp

Locked nucleic acid (LNA) probes from QIAGEN offer significantly higher thermal stability and mismatch discrimination than unmodified DNA, making them well suited for short-target detection in miRNA research, mRNA expression profiling by qPCR, SNP genotyping, and fluorescence in situ hybridization (FISH).

This custom manual service lets researchers specify their own target sequence while QIAGEN's team optimizes the placement of LNA residues for affinity and specificity. The oligo is synthesized with a 3' TAMRA (carboxytetramethylrhodamine) dye attached via a spacer arm, providing a red fluorophore with absorption near 557 nm and emission near 583 nm that pairs naturally with 5' FAM reporters in dual-label qPCR probes, or serves as a single-label reporter in custom hybridization assays.

Each order ships as a lyophilized pellet in a single tube, with recommended storage at -20 °C protected from light to preserve the TAMRA fluorophore and LNA modification integrity. Used in research applications including miRCURY LNA-based detection panels, custom PCR probes, and labeled capture oligos for microarray or pull-down workflows.