CRISPR-Q Sanger assay

An assay solution from QIAGEN designed for validating CRISPR edits by Sanger sequencing. It provides PCR primer sets and guidelines optimized for clean amplification of the target region, yielding sequence-ready products along with recommendations for sequencing and interpretation of edit outcomes.

The primers and protocol are optimized for Sanger-based validation of edits, and the package includes guidance for PCR setup and product cleanup prior to sequencing. The assay is intended for research use and should be validated within the user's own workflow prior to routine use.