CoV-2 ORF3a Pep Pool 25ug/pep

From STEMCELL Technologies, this lyophilized peptide pool is designed for immune cell activation. It is a mixture of 66 peptides derived from the open reading frame 3a (ORF3a) protein of SARS-CoV-2. The pool consists of 15-mer peptides with 11-amino-acid overlaps covering amino acids 1 - 275 of SARS-CoV-2 ORF3a. ORF3a is found in both SARS-CoV and SARS-CoV-2, and the two proteins share high sequence similarity. According to cited research, SARS-CoV ORF3a has been shown to cause apoptosis of infected cells (Ren et al., 2020), form potassium-sensitive ion channels that modulate virus release (Lu et al., 2006), and induce ligand-independent downregulation of the Type 1 interferon receptor (Minakshi et al., 2009).

The peptides are lyophilized as trifluoroacetate salts, supplied at approximately 25 μg per peptide, with an average purity of 70%. The source is SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2), with Protein ID P0DTC3, Gene Name 3a, and Protein Name Protein 3a; AP3A; ORF3a. The provided quantity (~25 μg/peptide) is sufficient for stimulating 2.5 x 10^8 cells. Store at -20°C; the product is stable as supplied until the expiry date (EXP) on the label. For preparation, warm to room temperature (15 - 25°C) before reconstitution, add pure dimethyl sulfoxide (DMSO; ~40 μL) and dilute with water to the desired concentration. Final DMSO concentration must be below 1% (v/v) to avoid toxicity in the biological system. If not used immediately, aliquot and store at -20°C, protect from light, and avoid repeated freeze-thaw cycles. According to the Safety Data Sheet, the chemical is not considered hazardous by the 2012 OSHA Hazard Communication Standard (29 CFR 1910.1200). Products are for research use only and not intended for human or animal diagnostic or therapeutic uses unless otherwise stated.