CMV pp65 Pep Pool 25ug/pep

STEMCELL Technologies' CMV (pp65) Peptide Pool is a lyophilized mixture of 138 peptides derived from the 65 kDa phosphoprotein (pp65) of human cytomegalovirus (CMV; strain AD169). The pool consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 - 561 on pp65 (Swiss-Prot Protein ID: P06725; Gene Name: UL83). It is supplied at approximately 25 μg/peptide, lyophilized as trifluoroacetate salts, with an average purity of 70%.

The peptide pool is designed to activate antigen-specific immune cells, making it suitable for research workflows involving serum-free and xeno-free media for T cell expansion, immunomagnetic negative isolation of untouched human T cells, and the use of primary human frozen cells. The pp65 protein has been studied for its role in counteracting the interferon response by preventing the activation of interferon response factor 3 (IRF-3), and for inducing transcription of the viral immediate-early promoter.

For preparation, the product should be warmed to room temperature (15 - 25°C) before reconstitution, dissolved in approximately 40 μL of pure dimethyl sulfoxide (DMSO), and diluted with water to the desired concentration. The final DMSO concentration must be kept below 1% (v/v) to avoid toxicity in biological systems. Store at -20°C, protected from light; the product is stable as supplied until the expiry date on the label. Products are for research use only and not intended for human or animal diagnostic or therapeutic uses.