ClonaCell-HY Pre-tested PEG, 1.5mL

Polyethylene glycol solutions serve as the fusogen at the heart of every hybridoma generation protocol, and this 1.5 mL format from STEMCELL Technologies delivers a pre-tested dose validated specifically for the ClonaCell-HY cell fusion workflow.

Each vial contains a single-fusion volume of PEG, pre-screened for fusion efficiency and hybridoma viability before release, so laboratories pursuing monoclonal antibody development avoid the time-consuming optimization that unvalidated PEG grades often require. The reagent is applied directly to a combined pellet of SP2/0 myeloma cells and freshly isolated splenocytes from immunized mice or rats, then diluted stepwise to drive membrane merger while limiting cytotoxic damage to the resulting hybrid cells.

Used alongside semi-solid cloning medium and HAT selection reagents in the broader ClonaCell-HY system, the PEG step bridges immunization and single-cell cloning in antibody discovery pipelines. Research use only applications that benefit include hybridoma cell line generation, immunogen-specific clone screening, and the production of monoclonal antibodies for downstream assay development. Refrigerate the vial and protect from light to preserve fusion activity through its stated shelf life.