Blood & Cell Culture DNA Midi Kit (25)

QIAGEN Blood & Cell Culture DNA Midi Kits provide gravity-flow, anion-exchange tips and buffers for efficient isolation of genomic DNA from a wide range of biological samples, without the use of phenol or chloroform. The kit enables reliable isolation of high-molecular-weight DNA up to 150 kb in size, with an average size of 50–100 kb, and supports convenient, parallel processing of multiple samples.

Using QIAGEN anion-exchange technology, the procedure is gentle and results in negligible DNA shearing. Purified DNA is free of contaminants such as RNA, protein and metabolites, with A260/A280 ratios between 1.7 and 1.9. The exceptionally large DNA size makes it especially suitable for preparing high-quality libraries for next-generation sequencing on different platforms. Hands-on time for the complete procedure is just 30 minutes for blood and cultured cells.

QIAGEN Blood & Cell Culture DNA Kits are well suited for applications including Sanger and next-generation sequencing, long-read sequencing, RFLP analysis, analysis of gene targeting, screening of transgenic animals, DNA fingerprinting studies, and PCR amplification. For yeast and bacteria samples, the Genomic DNA Buffer Set is required.