Biotin/Conjugate Goat Anti-Mu lgG, 125uL

The goat anti-mouse IgG, biotin-conjugated secondary antibody from STEMCELL Technologies is a versatile detection reagent for laboratories working with mouse-derived primary antibodies. Affinity-purified polyclonal antibodies raised in goats are covalently coupled to biotin, preserving binding activity toward mouse IgG heavy and light chains while allowing researchers to pair the reagent with their choice of streptavidin-based detection chemistry.

The 125 µL liquid vial format suits pilot studies, multiplex panels, and lower-throughput assays without committing to bulk volumes. Working dilutions commonly fall between 1:200 and 1:1,000 across applications, though optimal concentration depends on target abundance, sample preparation, and detection method. The biotin label supports chromogenic readouts combined with streptavidin-HRP, chemiluminescent detection on Western blot membranes, or fluorescent labeling via streptavidin-fluorophore conjugates for flow cytometry and immunofluorescence microscopy.

Uses include Western blot, immunohistochemistry on frozen or paraffin-embedded tissue sections, intracellular and cell-surface flow cytometry staining, and immunocytochemistry on cultured cells. For long-term stability, aliquot the reagent upon receipt and store frozen at -20 °C, protected from light. Active bench stocks can be kept refrigerated for several weeks; repeated freeze-thaw cycles reduce antibody performance over time.

Each vial contains carrier protein and stabilizers to maintain immunoglobulin conformation during storage and handling. End users should titrate the reagent against their own target and detection system before committing to scale-up workflows.