Anti-ZO-1 Antibody

Offered by Antibodies.com, this rabbit polyclonal reagent is raised against the tight-junction scaffolding protein ZO-1 (TJP1) and is supplied as 100 µl of an affinity-purified, unconjugated preparation. The immunogen corresponds to a recombinant fragment spanning amino acids 1–266 of the human TJP1 sequence, and the antibody is validated for Western blot (WB) and immunohistochemistry (IHC) on human samples, making it well suited to studies of epithelial barrier biology and junctional complexes. Because the host species is rabbit and the clone type is polyclonal, lot-to-lot consistency is supported by antigen-affinity chromatography rather than hybridoma cloning, and the product is provided in a liquid format — 0.1 M Tris-buffered saline at pH 7.0 supplemented with 10% glycerol and 0.01% thimerosal as a preservative — that is convenient for routine dilution into standard blocking and wash buffers. The conjugate status is unconjugated, so the reagent can be paired with a secondary detection system of the researcher's choice (HRP, biotin, or fluorophore-labelled anti-rabbit IgG), and it is shipped at 4°C with a recommended storage protocol of aliquoting upon arrival and freezing at –20°C while avoiding repeated freeze/thaw cycles, which preserves activity for long-term bench use. Intended for research-use-only applications and not for diagnostic or therapeutic work, this primary antibody from Antibodies.com gives laboratories a flexible tool for probing ZO-1 distribution and expression in cell monolayers, tissue sections, and lysate-based assays.