Anti-ZNF544 Antibody

Raised in rabbit and supplied by Antibodies.com, this polyclonal primary antibody recognizes ZNF544, a C2H2-type zinc finger transcription factor characterized in published studies for its testis-enriched expression pattern in humans. The unconjugated reagent is validated for Western blot and immunohistochemistry workflows and reacts specifically with the human target, supporting research into KRAB-domain zinc finger protein function and spermatogenesis.

The antibody is provided as a 100 µl aliquot at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, a formulation that drops directly into standard blotting and staining buffers. For long-term use, freeze the stock in single-use aliquots to limit freeze-thaw cycles and preserve binding activity; working solutions can be kept refrigerated for short-term use, and the glycerol also helps protect against freezing damage during routine handling.

Typical applications include Western blot detection of ZNF544 in human testis lysates, where the polyclonal reagent helps confirm the expected band size, and immunohistochemistry on fixed human tissue sections to map protein localization in seminiferous tubules. Investigators studying male germ cell development, transcriptional repression by KRAB-zinc finger proteins, and chromosome 19q13 loci have used affinity-purified polyclonal antibodies of this type to probe expression and to complement knockdown or overexpression experiments.