Anti-ZIP-kinase (K261) Antibody

ZIP-kinase (also known as DAPK3 or ZIPK) is a serine/threonine kinase belonging to the death-associated protein kinase family, implicated in apoptosis regulation, smooth muscle contraction, and cytoskeletal reorganization. This unconjugated rabbit polyclonal reagent from Antibodies.com is raised against a region of human ZIP-kinase centered around residue K261 and is validated for western blot (WB) and immunohistochemistry (IHC) workflows.

The antibody is supplied as a 50 µl vial at approximately 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2, ready for direct dilution into standard immunodetection buffers. Reactivity has been confirmed against human, mouse, and rat samples, supporting its use across common laboratory model systems for studies of stress-induced signaling, cytokinesis, and transcriptional regulation.

For western blot, the reagent is typically applied to whole-cell lysates prepared from cultured mammalian cells or fresh tissue homogenates, where it detects the endogenous kinase band. In IHC applications, it has been used on formalin-fixed paraffin-embedded tissue sections to localize ZIP-kinase expression in smooth muscle and other relevant cell types, including contractile and proliferating compartments.

Storage recommendations include refrigeration; aliquoting upon receipt helps limit freeze-thaw cycles and preserve antibody activity across multiple experiments. Researchers investigating DAP kinase family signaling, smooth muscle biology, or apoptosis pathways will find this polyclonal reagent a useful tool.