Anti-ZAP70 (phospho Y319) Antibody

A primary antibody from Antibodies.com, supplied as a rabbit polyclonal reagent that recognises ZAP70 when phosphorylated at Tyr319 (Y319). It is of the IgG isotype, unconjugated, and reacts with human samples. The target protein has a molecular weight of 70 kDa.

According to the manufacturer's specifications, the antibody is supplied in liquid form at a lot-specific concentration in Phosphate Buffered Saline (pH 7.3) with 50% glycerol and 0.05% Proclin 300, and has been affinity purified. It is shipped at 4°C and upon receipt should be aliquoted and stored at -20°C, avoiding repeated freeze/thaw cycles.

The antibody was raised against a synthetic phosphorylated peptide around Y319 of human ZAP70 (NP_001070.2) with immunogen sequence SPYSD. Recommended dilutions are 1:500-1:2,000 for WB and 1:50-1:100 for IP. The catalogue lists applications including WB, IHC, IF and IP, with the manufacturer confirming validation for WB and IP.