Anti-YWHAE Antibody

YWHAE encodes the 14-3-3 epsilon scaffolding protein, a ~29 kDa member of the conserved 14-3-3 family that binds phosphoserine and phosphothreonine motifs to regulate cell signaling, apoptosis, and neuronal migration. This unconjugated rabbit polyclonal reagent from Antibodies.com is supplied as 100 µl of antiserum validated for western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) on human and mouse samples.

The antibody detects endogenous 14-3-3 epsilon in lysates and tissue sections, supporting studies of brain development (YWHAE haploinsufficiency is linked to Miller-Dieker syndrome), glioma biology, and signaling pathway analysis. Polyclonal reactivity across multiple epitopes improves tolerance to minor conformational changes in the target, which is helpful when probing denatured samples.

Shipped as a single vial and stored refrigerated between uses, the unconjugated format allows flexible pairing with HRP-, biotin-, or fluorophore-conjugated secondary reagents matched to the chosen detection method. Brief centrifugation recovers full volume before each use.