Anti-YARS2 Antibody

Mitochondrial protein synthesis depends on a dedicated set of nuclear-encoded aminoacyl-tRNA synthetases, including YARS2, which charges mitochondrial tRNA-Tyr with tyrosine, and a rabbit polyclonal antibody from Antibodies.com enables immunological detection of this enzyme in human samples. The unconjugated Anti-YARS2 Antibody reagent is supplied as 100 µl of polyclonal antiserum and is validated for Western blot and immunohistochemistry applications, with the unconjugated format giving researchers flexibility to pair it with their preferred secondary labels.

Host species is rabbit, clonality is polyclonal, isotype is IgG, and the reagent is intended for research use only. Typical Western blot working dilutions range from 1:500 to 1:2000 depending on tissue abundance and detection chemistry, while IHC on formalin-fixed paraffin-embedded sections is generally performed at 1:100 to 1:500 following heat-induced antigen retrieval. Loss-of-function mutations in YARS2 are associated with MLASA2 (mitochondrial myopathy, lactic acidosis and sensorineural hearing loss type 2), so reliable detection of the enzyme supports studies of mitochondrial translation, respiratory chain assembly, and related disease pathogenesis.

For handling, keep the liquid antibody refrigerated at 4 °C for short-term use and store working aliquots frozen at -20 °C for longer-term stability, limiting freeze-thaw cycles and protecting from light to preserve activity. The unconjugated format also accommodates downstream labelling for immunofluorescence, ELISA, or affinity purification workflows commonly used by researchers studying mitochondrial biology.