Anti-YAP1 Antibody [Assigned #A11326]

YAP1 (Yes-Associated Protein 1) is a transcriptional coactivator and central effector of the Hippo tumor-suppressor pathway, regulating organ size, mechanotransduction, and contact inhibition through TEAD-dependent gene programs. Nuclear translocation of YAP1 is a widely used readout of Hippo pathway activity in cell biology and oncology research, and this unconjugated rabbit monoclonal IgG primary antibody from Antibodies.com provides a reproducible reagent for tracking that event across experimental systems.

Supplied as 200 µl of ready-to-use reagent, the antibody is validated for western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP) workflows performed on human samples. The monoclonal IgG format delivers consistent epitope recognition across experiments, supporting reliable detection of endogenous YAP1 in cell lysates, formalin-fixed paraffin-embedded tissue sections, and fixed immunofluorescence preparations without the lot-to-lot variability typical of polyclonal reagents.

Investigators apply this reagent to study Hippo signaling dynamics, YAP/TAZ-TEAD mechanosensing, and downstream transcriptional programs in epithelial, hepatic, neuronal, and cancer model systems, where YAP1 status is frequently correlated with proliferation, migration, and differentiation phenotypes. For routine use, short-term storage at 4 °C is appropriate; for longer-term storage, aliquot and freeze at -20 °C and protect from light to preserve activity across repeated freeze-thaw cycles. The product is supplied for research use only in in vitro applications.