Anti-XRCC4 Antibody

This rabbit polyclonal IgG antibody from Antibodies.com specifically detects human XRCC4, a core scaffolding component of the non-homologous end joining (NHEJ) pathway that repairs DNA double-strand breaks. XRCC4 forms a stabilizing heterodimer with DNA Ligase IV (LIG4) through its C-terminal region, positions the ligase on broken DNA ends, and is essential for V(D)J recombination during lymphocyte receptor gene assembly. The unconjugated reagent is supplied as 200 µl of liquid stock in a vial and is validated for Western blot detection of the endogenous protein in human cell lysates. Aliquot upon receipt and store at -20 °C; avoid repeated freeze-thaw cycles to preserve binding activity. Researchers studying genomic stability, DNA damage response signaling, radiosensitivity, class switch recombination, or lymphoid development will find this antibody useful as a probe for endogenous XRCC4 levels, particularly alongside phospho-specific markers such as γH2AX, KAP1, or ATM substrates that report on the same double-strand break response.