Anti-XRCC4 Antibody

XRCC4 (X-Ray Repair Cross Complementing 4) is a nuclear scaffolding protein central to the canonical non-homologous end joining (NHEJ) pathway that ligates DNA double-strand breaks in human cells. This polyclonal reagent from Antibodies.com is raised in rabbit, isotype IgG, unconjugated, and validated for Western blot detection of the human target. Each vial is supplied as 100 µl of antibody; for short-term storage keep refrigerated at 4°C, and for longer-term storage keep frozen at -20°C, protected from light and from repeated freeze-thaw cycles to preserve binding activity.

The antibody is commonly deployed in DNA damage response studies, including work on ionizing radiation sensitivity, V(D)J recombination in developing lymphocytes, and the mechanisms by which tumour cells survive genotoxic chemotherapy. Because XRCC4 partners with DNA Ligase IV, Ku70, Ku80, and XLF within the repair complex, it is a useful marker when comparing NHEJ-proficient and NHEJ-deficient cell lines, and it is frequently cited in studies of severe combined immunodeficiency phenotypes linked to XRCC4 loss-of-function mutations.

For Western blot, resolve lysates from cultured human cells under standard reducing SDS-PAGE conditions, transfer to nitrocellulose or PVDF, and detect with an HRP- or fluorescence-conjugated secondary reagent. The expected band is in the ~38-55 kDa region depending on isoform and post-translational state. This product is intended for research use only and is not for diagnostic or therapeutic application.