Anti-XRCC3 Antibody

The Antibodies.com polyclonal antibody to XRCC3 is raised in rabbit and supplied as an unconjugated reagent for laboratories studying DNA double-strand break repair and homologous recombination. XRCC3 is a RAD51 paralog that participates in the RAD51C-containing complex during strand invasion and Holliday junction processing, making this reagent useful for work on genomic stability, radiation and chemotherapeutic response, and tumor susceptibility models. The 100 µL vial format at this pack size supports multiple western blot, immunohistochemistry, and immunofluorescence runs against human and mouse samples without immediate re-ordering.

Validated applications cover western blot for resolving the ~38 kDa XRCC3 band in cell lysates, immunohistochemistry on paraffin sections for tissue-level localization in tumor and matched normal specimens, and immunofluorescence for visualizing subnuclear foci that form at sites of DNA damage. Reactivity is established for human and mouse, supporting comparative studies in patient-derived material and murine knockout or genotoxin-challenge models. The unconjugated format lets each laboratory pair the antibody with a chosen HRP, biotin, or fluorophore secondary, which is convenient for groups running both chromogenic and fluorescence workflows on the same target.

For handling, keep the vial refrigerated and aliquot upon first opening if the reagent will be used intermittently over weeks, avoiding repeated freeze-thaw cycles. Brief centrifugation before use recovers material from the cap and sides of the tube. As with all affinity reagents, titrate against the specific sample preparation in use, since fixation, antigen retrieval, and blocking conditions shift the working concentration needed for clean signal in a given protocol.