Anti-XPB Antibody

This unconjugated rabbit polyclonal reagent from Antibodies.com targets XPB, the ERCC3-encoded 5' to 3' DNA helicase that forms part of the human TFIIH transcription and DNA repair complex. Supplied as a 100 µl vial and validated for western blot and immunofluorescence, the antibody is widely used in studies of nucleotide excision repair and RNA polymerase II transcription initiation. XPB mutations underlie xeroderma pigmentosum, Cockayne syndrome, and trichothiodystrophy, making this reagent a common tool for characterizing patient-derived fibroblasts and repair-deficient cell lines. Researchers compare lysate banding patterns from UV-irradiated versus untreated cells and rely on the antibody to assess TFIIH subunit integrity during chromatin remodeling assays. For storage, aliquot upon arrival, keep at -20 °C, and limit freeze-thaw cycles to preserve binding affinity over extended use.