Anti-WRB Antibody

Generated in rabbit as an unconjugated polyclonal IgG, this Antibodies.com primary antibody recognizes the tryptophan-rich basic protein WRB, also known as congenital heart disease 5 protein (CHD5), which is encoded on human chromosome 21q22.3. Each vial contains 200 µL of liquid antiserum ready for direct dilution into immunoblotting and chromogenic IHC workflows. Validated reactivity across human, mouse, and rat samples makes the reagent broadly applicable to comparative studies of mitochondrial protein import, GET-pathway function, and tail-anchored protein biogenesis.

The reagent detects endogenous WRB in cell culture lysates and frozen tissue sections by western blot (WB) and immunohistochemistry (IHC). WRB serves as a subunit of the TIM23 translocase and partners with GET4 to mediate insertion of tail-anchored proteins into the endoplasmic reticulum membrane, so investigators working on mitochondrial biogenesis, congenital heart disease genetics, and post-translational targeting routinely deploy this antibody to confirm expression and subcellular distribution. Store at -20 °C and protect from repeated freeze-thaw cycles to preserve performance over multiple experiments. The unconjugated IgG format also permits pairing with HRP-, biotin-, or fluorophore-labeled secondary antibodies, supporting flexible chromogenic, chemiluminescent, or fluorescence detection downstream.