Anti-WNK3 Antibody

Raised in rabbit against a synthetic peptide derived from human WNK lysine-deficient protein kinase 3, this unconjugated polyclonal IgG reagent from Antibodies.com is antigen-affinity purified and supplied as 100 µl of liquid antisera in pH 7.4 PBS containing 0.05% sodium azide and 40% glycerol.

The reagent is validated for Western blot and immunohistochemistry in lysates and fixed sections from human, mouse, and rat tissue, detecting endogenous total WNK3 protein. WNK3 sets the phosphorylation state of cation-chloride cotransporters NKCC1, NKCC2, NCC, KCC1, and KCC2 through SPAK/OSR1 signaling, and the kinase is enriched in neurons of the hippocampus, dentate gyrus, supraoptic and suprachiasmatic nuclei, locus ceruleus, and the Purkinje layer of the cerebellum, where it colocalizes with GABA_A receptor-expressing cells that control intracellular chloride and GABAergic tone.

Shipped at 4°C, the vial should be aliquoted on arrival and stored at -20°C to avoid repeated freeze-thaw cycles; pairing with a rabbit-compatible secondary of choice enables standard chromogenic or immunofluorescence readouts in immunoblot and IHC workflows.