Anti-WNK2 Antibody

WNK2 belongs to the WNK (With No Lysine) family of serine/threonine kinases that govern ion transport and cellular signaling. This rabbit polyclonal antibody from antibodies.com is raised against WNK2 and is validated for western blot detection of the human, mouse, and rat protein. The unconjugated reagent is supplied as 100 µl of a 1 mg/ml solution in phosphate buffered saline (pH 7.3) containing 0.05% sodium azide as a preservative.

Researchers investigating sodium and chloride homeostasis, NCC and NKCC transporter regulation, and signaling through the STE20-related kinase pathway rely on anti-WNK2 to probe kinase expression in renal epithelial models, glioma cell lines, and neural tissue where WNK2 has been reported to act as a tumor suppressor. The polyclonal format recognizes multiple epitopes on the target, useful when probing splice variants or post-translational modifications.

For western blot applications, dilute the antibody in blocking buffer and incubate with transferred membranes; expected band sizes align with a predicted WNK2 molecular weight near 230 kDa depending on isoform. Refrigerate the vial upon arrival; for long-term storage, aliquot and freeze at -80 degrees C to limit freeze-thaw cycles and preserve activity. The PBS-based formulation is ready to use after brief equilibration to room temperature.