Anti-WNK1 Antibody

WNK1 (With No Lysine Kinase 1) is a serine/threonine kinase that regulates ion transport, particularly sodium and chloride handling in the kidney distal nephron. Mutations in this kinase are linked to pseudohypoaldosteronism type II (Gordon syndrome), making the protein a target of interest for cardiovascular and renal physiology research.

This unconjugated rabbit polyclonal reagent from Antibodies.com is raised against WNK1 and is validated for Western blot, immunohistochemistry, and immunofluorescence workflows across Human, Mouse, and Rat samples. The polyclonal format provides recognition of multiple epitopes on full-length WNK1, which can be useful when probing tissue-specific expression differences between the widely expressed L-WNK1 isoform and the kidney-enriched KS-WNK1 variant.

The antibody is supplied as 100 µl of liquid, unconjugated, allowing labs to pair it with their own secondary antibody and detection chemistry for fluorescence, chromogenic, or chemiluminescent readouts. Its cross-species reactivity makes it a practical option for comparative studies in rodent disease models and human clinical samples, including kidney, brain, and cardiac tissue where WNK1 signaling has documented roles.

Typical experimental uses include confirming WNK1 expression changes in response to dietary salt manipulation, mapping kinase distribution in kidney sections by immunohistochemistry, and tracking WNK1 localization at the cellular level by immunofluorescence in cultured cells.