Anti-WIPI2 Antibody

The WIPI2 protein (WD repeat domain, phosphoinositide-interacting protein 2) is a phosphatidylinositol 3-phosphate effector that localizes to the nascent phagophore, where it scaffolds the lipidation machinery responsible for converting LC3-I to its autophagosome-associated LC3-II form during autophagy induction. This unconjugated rabbit polyclonal IgG reagent from Antibodies.com is raised against WIPI2 and supplied in a 100 µL volume in a screw-cap vial, ready to dilute into standard blocking buffers for immunohistochemistry and western blot workflows. In fixed tissue sections, the antibody yields a characteristic cytoplasmic and perinuclear staining pattern consistent with WIPI2's known localization to autophagosome precursors and, in some specialized tissues, to secretory granule compartments. Western blot detection of endogenous WIPI2 produces a band near the predicted molecular mass of approximately 49 kDa, supporting quantitative monitoring of autophagy induction and progression in human and murine cell culture lysates and tissue research samples. Investigators working on mitophagy, selective autophagy pathways, and autophagy-related disease models commonly use this polyclonal primary antibody for routine detection and side-by-side comparison across panels of human and mouse specimens.