Anti-VPS24 Antibody

This polyclonal antibody from Antibodies.com targets VPS24, also known as CHMP3, a core subunit of the ESCRT-III complex governing multivesicular body biogenesis, membrane scission at late endosomes, and the final abscission step of cytokinesis. The immunogen-derived reagent is raised in a rabbit host and purified as an IgG immunoglobulin, supplied unconjugated in a 200 µl volume suitable for laboratory use.

Validated for western blot, the reagent detects endogenous VPS24 in lysates prepared from human and mouse cell lines and primary tissue. Researchers studying HIV budding, intraluminal vesicle formation, endosomal sorting, autophagy, and membrane remodeling pathways rely on this reagent to monitor ESCRT-III assembly and disassembly dynamics. The unconjugated format allows flexible secondary antibody selection, including fluorescent, HRP, or biotin conjugates depending on downstream detection needs.

For best results, keep the antibody refrigerated upon receipt and avoid repeated freeze-thaw cycles; aliquot upon first use if extended storage is needed. The polyclonal nature of the reagent provides recognition of multiple epitopes on the VPS24 target, increasing tolerance for minor protein variations across experimental conditions and supporting reproducible band detection across biological replicates.