Anti-Vimentin Antibody

Manufactured by Antibodies.com, this Rabbit polyclonal antibody targets Vimentin and is supplied at the 50 µl size as an unconjugated, affinity-purified reagent at a concentration of 1 mg/ml. The immunogen is a synthetic peptide corresponding to amino acids 452–456 (G-Q-V-I-N) of human Vimentin, enabling detection of endogenous total Vimentin protein with a predicted molecular weight of 57 kDa. Recommended applications include Western blotting (1:500–1:1000) and Immunofluorescence (1:100–1:200), and the antibody has been confirmed to react with Human, Mouse, Rat, and Monkey samples, making it a versatile reagent for cross-species cytoskeletal studies. The product is formulated in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, 150 mM NaCl, 0.02% sodium azide, and 50% glycerol, and is shipped on blue ice for stability; long-term storage at –20 °C is recommended, while working aliquots may be kept at 4 °C for short-term use. Produced by immunizing rabbits with the peptide–KLH conjugate and purified by epitope-specific affinity chromatography, this reagent is intended for research use only and is not for diagnostic or therapeutic applications.