Anti-Vimentin Antibody

Raised in rabbit as an unconjugated polyclonal reagent, this primary antibody targets vimentin, a type III intermediate filament protein widely used as a marker of mesenchymal lineage cells and as a reporter for epithelial-to-mesenchymal transition in cancer and developmental biology studies. Validated for Western blot and immunofluorescence workflows, the antibody cross-reacts with human, mouse, rat, and monkey samples, allowing comparative work across cell lines and primary tissues.

Supplied as 100 µl of liquid, the reagent supports research use in confirming the mesenchymal character of cultured cells, verifying fibroblast contamination in neural or epithelial cultures, characterizing circulating tumor cells, and monitoring cytoskeletal remodeling in response to pharmacological treatments. Researchers working on sarcoma subtyping, fibrosis models, wound healing assays, and stem cell differentiation protocols also rely on vimentin detection as a routine quality checkpoint.

Because vimentin is a structural protein of approximately 57 kDa, band patterns on Western blot at the expected molecular weight provide a useful internal control alongside loading markers, while immunofluorescence visualization highlights the filamentous network extending from the perinuclear region throughout the cytoplasm. Antibodies.com prepares this polyclonal antibody under standard quality control procedures to support reproducible results in cell biology and translational research laboratories.